Jinhua Dong


Jinhua Dong




  • 提示技術による新型抗体の開発及び応用

  • Open Sandwich免疫測定法及びQuenchbody免疫蛍光プローブの研究








東京工業大学 資源化学研究所 助教


臨沂大学化学化工学院 教授


東京工業大学科学技術創成研究院 特任教授
Weifang Medical University, School of Bioscience and Technology Professor


Excellent Paper Award of The Society for Biotechnology, Japan




Dong, J., Miyake, C., Yasuda, T., Oyama, H., Morita, I., Tsukahara, T., Takahashi, M., Jeong, H.J., Kitaguchi, T., Kobayashi, N., and Ueda, H., PM Q-probe: a fluorescent binding protein that converts many antibodies to a fluorescent biosensor, Biosens. Bioelectron, 165,112425, 2020

Dong, J., Oka, Y., Jeong, H.J., Ohmuro-Matsuyama, Y., and Ueda, H., Detection and destruction of HER2-positive cancer cells by Ultra Quenchbody-siRNA complex, Biotechnol. Bioeng., 117, 1259-1269, 2020


Cong, Y., Dong, H., Wei, X., Zhang, L., Bai, J., Wu, J., Huang, X.J., Gao, Z., Ueda, H. and Dong, J., A novel murine antibody and an Open sandwich immunoassay for the detection of clenbuterol, Ecotoxicol. Environ. Saf., 182,109473, 2019

Gong, Z., Liu, X., Dong, J., Zhang, W., Jiang, Y., Zhang, J., Feng, W., Chen, K., Bai, J., Transition from vesicles to nanofibres in the enzymatic self-assemblies of an amphiphilic peptide as an antitumour drug carrier, Nanoscale, 11(33):15479-15486, 2019


Dong, J., Fujita, R., Zako, T., Ueda, H., Construction of Quenchbodies to detect and image Amyloid β oligomers, Anal. Biochem., 550,61-67, 2018

Zhao, S., Dong, J., Jeong, H.J., Okumura, K., Ueda, H., Rapid detection of the neonicotinoid insecticide imidacloprid using a quenchbody assay, Anal. Bioanal. Chem., 410(17),4219-4226, 2018

Su, J., Dong, J., Kitaguchi, T., Ohmuro-Matsuyama, Y., Ueda, H., Noncompetitive homogeneous immunodetection of small molecules based on β-glucuronidase complementation, Analyst, 143, 2096-2101, 2018


Dong, J., Shichiri, M., Chung, C.I., Shibata, T., Uchida, K., Hagihara, Y., Yoshida, Y., Ueda, H., An Open Sandwich immunoassay for detection of 13(R,S)-Hydroxy-9(E),11(E)-octadecadienoic acid, Analyst, 142(5), 787-793, 2017

Dong, J., Ueda, H., ELISA-type assays of trace biomarkers using microfluidic methods, WIREs Nanomed. Nanobiotechnol., e1457, 2017

Wongso, D., Dong, J., Ueda, H., Kitaguchi, T., Flashbody: a next generation Fluobody with fluorescence intensity enhanced by antigen binding, Anal. Chem., 89(12),6719-6725, 2017

Jeong, H.J., Kawamura, T., Iida, M., Kawahigashi, Y., Takigawa, M., Ohmuro-Matsuyama, M., Chung, C.I., Dong, J., Kondoh, M., Ueda, H., Development of a Quenchbody for the detection and imaging of the cancer-related tight-junction-associated membrane protein claudin, Anal Chem., 89(20),10783-10789, 2017


Dong, J., Jeong, H.J., Ueda, H., Preparation of Quenchbodies by protein transamination reaction, J. Biosci. Bioeng., 122(1), 125-130, 2016


Dong, J., Kojima, T., Ohashi H., Ueda, H., Optimal fusion of antibody binding domains resulted in higher affinity and wider specificity, J. Biosci. Bioeng., 120(5),504-9, 2015

Chung, C.I., Makino, R., Dong, J., Ueda, H., Open flower fluoroimmunoassay: a general method to make fluorescent protein-based immunosensor probes, Anal. Chem., 87(6), 3513-9, 2015


Dong, J., Otsuki, T., Kato, T., Park, E.Y., Tracking Neospora caninum parasites using chimera monoclonal antibodies against its surface antigen-related sequences (rNcSRS2), J. Biosci. Bioeng., 117(3), 351-357, 2014


Zhou, H.*, Dong, J.*, Deo, V.K., Park, E.Y., Lee, J. Detection of anti-Neospora antibodies in bovine serum by using spiky Au-CdTe nanocomplexes, Sens. actuators. B Chem., 178,192-199 (2013) *equal contributions

Dong, J., Sakurai, A., Nomura, N., Park, E.Y., Shibasaki, F., Ueda, H., Isolation of recombinant phage antibodies targeting the hemagglutinin cleavage site of highly pathogenic avian influenza virus, PloS One, 8(4), e61158, 2013


Dong, J., Otsuki, T., Kato, T., Park, E.Y., Development of a diagnostic method for neosporosis in cattle using recombinant Neospora caninum proteins, BMC Biotechnol., 12,19, 2012


Islam, K., Ihara, M., Dong, J., Kasagi, N., Mori, T., Ueda, H., Direct construction of open-sandwich enzyme immunoassay for one-step noncompetitive detection of thyroid hormone T4, Anal. Chem., 83(3),1008-1014, 2011


Dong, J., Ihara, M., Ueda, H., Antibody Fab display system that can perform open-sandwich ELISA, Anal. Biochem., 386(1),36-44, 2009


Dong, J., Tamaru, Araki, T., Molecular cloning, expression, and characterization of a β-agarase gene, agaD, from a marine bacterium, Vibrio sp. strain PO-303, Biosci. Biotechnol. Biochem., 71(1),38-46, 2007

Dong, J., Tamaru, Y., Araki, T., A unique β-agarase, AgaA, from a marine bacterium, Vibrio sp. strain PO-303, Appl. Microbiol. Biot., 74(6),1248-1255, 2007


Dong, J., Hashikawa, S., Konishi, T., Tamaru, Y., Araki, T., Cloning of the novel gene encoding β-Agarase C from a marine bacterium, Vibrio sp. strain PO-303, and characterization of the gene product, Appl. Environ. Microbiol., 72(9),6399-6410, 2006