Jinhua Dong

Cell Biology

Jinhua Dong

Specially Appointed Professor

genetic EngineeringProtein EngineeringAntibody EngineeringImmunoassay

Research Projects

  • Development and application of novel antibodies based on display technologies

  • Studies on Open Sandwich Immunoassay and Quenchbody technology


The University of Tokyo, Research Fellow


JSPS Fellowship (The University of Tokyo)


Shizuoka University, Researcher, Assistant Prof.


Tokyo Institute of Technology, Assistant Prof.


Linyi University, School of Chemistry, Professor


Tokyo Institute of Technology, Specially Appointed Professor
Weifang Medical University, School of Bioscience and Technology Professor


Chinese Government Award for Outstanding Students Abroad


Fuji Sankei Business i, The 29th Originality innovation, High-Technology Award


Excellent Paper Award of The Society for Biotechnology, Japan



Dong, J., Hashikawa, S., Konishi, T., Tamaru, Y., Araki, T., Cloning of the novel gene encoding β-Agarase C from a marine bacterium, Vibrio sp. strain PO-303, and characterization of the gene product, Appl. Environ. Microbiol., 72(9),6399-6410 (2006)


Dong, J., Tamaru, Araki, T., Molecular cloning, expression, and characterization of a β-agarase gene, agaD, from a marine bacterium, Vibrio sp. strain PO-303, Biosci. Biotechnol. Biochem., 71(1),38-46 (2007)

Dong, J., Tamaru, Y., Araki, T., A unique β-agarase, AgaA, from a marine bacterium, Vibrio sp. strain PO-303, Appl. Microbiol. Biot., 74(6),1248-1255 (2007)


Dong, J., Ihara, M., Ueda, H., Antibody Fab display system that can perform open-sandwich ELISA, Anal. Biochem., 386(1),36-44 (2009)


Islam, K., Ihara, M., Dong, J., Kasagi, N., Mori, T., Ueda, H., Direct construction of open-sandwich enzyme immunoassay for one-step noncompetitive detection of thyroid hormone T4, Anal. Chem., 83(3),1008-1014 (2011)



Dong, J., Otsuki, T., Kato, T., Park, E.Y., Development of a diagnostic method for neosporosis in cattle using recombinant Neospora caninum proteins, BMC Biotechnol., 12,19 (2012)


Zhou, H.,* Dong, J.,* Deo, V.K., Park, E.Y., Lee, J. Detection of anti-Neospora antibodies in bovine serum by using spiky Au-CdTenanocomplexes, Sens. actuators. B Chem., 178,192-199 (2013) *equal contributions

Dong, J., Sakurai, A., Nomura, N., Park, E.Y., Shibasaki, F., Ueda, H., Isolation of recombinant phage antibodies targeting the hemagglutinin cleavage site of highly pathogenic avian influenza virus, PloS One, 8(4), e61158 (2013)


Dong, J., Otsuki, T., Kato, T., Park, E.Y., Tracking Neospora caninum parasites using chimera monoclonal antibodies against its surface antigen-related sequences (rNcSRS2), J. Biosci. Bioeng., 117(3), 351-357 (2014)


Dong, J., Kojima, T., Ohashi H., Ueda, H., Optimal fusion of antibody binding domains resulted in higher affinity and wider specificity, J. Biosci. Bioeng., 120(5),504-9 (2015)

Chung, C.I., Makino, R., Dong, J., Ueda, H., Open flower fluoroimmunoassay: a general method to make fluorescent protein-based immunosensor probes, Anal. Chem., 87(6), 3513-9 (2015)


Dong, J., Jeong, H.J., Ueda, H., Preparation of Quenchbodies by protein transamination reaction, J. Biosci. Bioeng., 122(1), 125-130 (2016)



Dong, J., Shichiri, M., Chung, C.I., Shibata, T., Uchida, K., Hagihara, Y., Yoshida, Y., Ueda, H., An Open Sandwich immunoassay for detection of 13(R,S)-Hydroxy-9(E),11(E)-octadecadienoic acid, Analyst, 142(5), 787-793 (2017)

Dong, J., Ueda, H., ELISA-type assays of trace biomarkers using microfluidic methods, WIREs WIREs Nanomed. Nanobiotechnol., e1457 (2017)

Wongso, D., Dong, J., Ueda, H., Kitaguchi, T., Flashbody: a next generation Fluobody with fluorescence intensity enhanced by antigen binding, Anal. Chem., 89(12),6719-6725 (2017)

Jeong, H.J., Kawamura, T., Iida, M., Kawahigashi, Y., Takigawa, M., Ohmuro-Matsuyama, M., Chung, C.I., Dong, J., Kondoh, M., Ueda, H., Development of a Quenchbody for the detection and imaging of the cancer-related tight-junction-associated membrane protein claudin, Anal Chem. 89(20),10783-10789 (2017)


Dong, J., Fujita, R., Zako, T., Ueda, H., Construction of Quenchbodies to detect and image Amyloid β oligomers, Anal. Biochem., 550,61-67 (2018)

Zhao, S., Dong, J., Jeong, H.J., Okumura, K., Ueda, H., Rapid detection of the neonicotinoid insecticide imidacloprid using a quenchbody assay, Anal. Bioanal. Chem., 410(17),4219-4226, (2018)

Su, J., Dong, J., Kitaguchi, T., Ohmuro-Matsuyama, Y., Ueda, H., Noncompetitive homogeneous immunodetection of small molecules based on β-glucuronidase complementation, Analyst, 143, 2096-2101 (2018)


Cong, Y., Dong, H., Wei, X., Zhang, L., Bai, J., Wu, J., Huang, J.X., Gao, Z., Ueda, H., Dong, J., A novel murine antibody and an Open sandwich immunoassay for the detection of clenbuterol, Ecotoxicol. Environ. Saf., (2019), doi: 10.1016/j.ecoenv.2019.109473

Gong, Z., Liu, X., Dong, J., Zhang, W., Jiang, Y., Zhang, J., Feng, W., Chen, K., Bai, J., Transition from vesicles to nanofibres in the enzymatic self-assemblies of an amphiphilic peptide as an antitumour drug carrier, Nanoscale, (2019). doi: 10.1039/c9nr02874a